Fabrication of a Circular Pdms Microchannel to Construct 3d Culture Microsystem

نویسندگان

  • Jong Seob Choi
  • Yunxian Piao
  • Tae Seok Seo
چکیده

In this study, we described the fabrication method for microfluidic channels with circular-cross sectional geometries by reflow phenomenon. The photoresist reflow leads to shape change from rectangular to semicircular, and we optimized the reflow conditions by using positive photoresist to form the perfect cylindrical micropatterns. The resultant circular micropattern was used as a template for generating PDMS circular microchannels. The channel routes could be easily modified for mimicking the vascular network. To generate a 3D endothelial cell layer, we cultured the HUVECs inside the PDMS circular microchannels, and monitored the adhesion, proliferation, and alignment of HUVEC to the direction of the microchannel.

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تاریخ انتشار 2012